FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13911735
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC573110.4119615561969805TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68500.060.6105546
CGTATGC75050.055.41822444
GTATGCC78600.053.1364245
ATGCCGT78500.052.84410547
TCGTATG83250.049.9173843
TGCCGTC85150.048.84044648
GCCGTCT90350.045.98875449
CTCGTAT90700.045.3905142
ACTGACC93800.044.36760332
CACTGAC95550.043.66490631
ACGTCTG97450.043.13092815
AGTCACT107300.039.27675228
ACACGTC107300.039.20234713
CACACGT107850.039.09977312
GCACACG108800.038.79053511
TCTCGTA108300.038.07744241
CGTCTGA115800.036.32741516
GTCACTG116400.036.17555629
CACGTCT116650.036.15037514
AATCTCG123850.033.5217939
Produced by FastQC (version 0.11.5)