FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610378
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT225310.1786702983843942TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145010.1149925878510541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32450.050.59374648
CGTACGT34150.048.07021334
CCGTACG37200.044.22376633
GTATGCC37700.044.01281747
GTACGTA37650.043.60274535
CGTATGC39450.041.7061346
ATGCCGT42900.038.8395949
TACGTAA44100.037.2263636
TCGTATG44850.036.68567345
ACGTCTG48250.035.178715
TGCCGTC50750.033.106650
ACCGTAC50400.032.9190232
CTCGTAT51500.031.88074344
GCACACG54500.031.20766811
GCCGTCT53500.031.0771151
ACACGTC56750.030.03155513
CACACGT60750.027.99711612
ACGTAAT59300.027.86166637
CACGTCT67200.025.41354614
CGTCTGA69100.024.96957416
Produced by FastQC (version 0.11.5)