FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11669628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC607020.520170822925975TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG73050.059.90457546
GTATGCC77300.056.7943345
CGTATGC78250.056.19550344
ATGCCGT82450.053.03203247
TCGTATG83100.052.91551243
CTCGTAT87450.049.6014942
TGCCGTC89950.048.53156748
ACGTCTG94000.047.5063415
GCCGTCT96650.045.16453649
GCACACG104300.042.98010611
ACACGTC104100.042.894613
TCTCGTA103050.042.1252141
GTCACCT105900.042.0770229
CACACGT106700.041.81675312
CACGTCT109850.040.90400314
CGTCTGA111750.040.0870616
AGCACAC117150.038.23581310
AATCTCG116500.037.26134539
CAGTCAC120800.036.85886827
ATCTCGT117900.036.72996540
Produced by FastQC (version 0.11.5)