FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bur-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13347389
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC246670.18480768036355275TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33550.051.96378746
GTATGCC41100.042.76091845
CGTATGC41900.042.02882444
TCGTATG47150.037.12615243
ATGCCGT46850.036.9131747
CTCGTAT51550.033.75341442
TGCCGTC52700.033.14736648
ACTTAGG56050.031.53563532
CACTTAG58350.030.4117631
TCACTTA59150.030.05916230
GCCGTCT58600.029.8078849
TTAGGCA61900.029.29059634
TAGGCAT62300.029.27305835
AGTCACT70400.025.45730628
ACGTCTG72400.024.94306215
TCTCGTA71600.024.59369741
CTTAGGC73850.024.17196833
GTCACTT75600.023.93769329
GGCATCT74100.023.9039837
AGGCATC80950.022.61602436
Produced by FastQC (version 0.11.5)