FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506178
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT285440.24807542521939085TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164020.1425495068823027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35650.055.38356448
CGTATGC42300.046.926446
GTATGCC43950.045.16406647
CGTGGCC44050.045.13414433
ATGCCGT44900.044.2048349
TCGTATG47950.041.3989945
GCCTTAT48450.040.9649937
ACGTGGC48750.040.92599532
CACGTGG51100.039.2481831
TATCTCG50650.039.19008641
ACGTCTG53350.038.44029615
TGCCGTC52300.037.94841450
GTCACGT52900.037.6506629
CTCGTAT54300.036.81653644
GCACACG56050.036.6492611
GCCGTCT55700.035.81958851
TCACGTG56750.035.09333830
CACACGT59250.034.78832212
GTGGCCT57500.034.75809534
AGTCACG57900.034.5203728
Produced by FastQC (version 0.11.5)