FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14681968
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1746651.1896565909965204TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG197250.064.550246
GTATGCC204500.062.77852245
CGTATGC204250.062.4806144
ATGCCGT205700.061.21651547
TCGTATG206000.060.65814243
TGCCGTC210250.059.50755348
CTCGTAT214750.058.67392742
TATCTCG217050.058.59760339
GCCGTCT216200.057.02535249
CACTAGC244050.055.5595731
ACTAGCT248550.054.56979432
TAGCTTA248250.054.5509634
GTCACTA249300.054.44904329
ACGTCTG252750.054.18037815
GCTTATC248300.054.1641636
AGTCACT252050.054.02254528
TCACTAG251700.053.9117330
TCTCGTA236400.053.16534841
AGCTTAT261600.051.75707235
CACACGT265250.051.71712
Produced by FastQC (version 0.11.5)