FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Bl-1_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208147
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC279360.2736637707117658TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34000.057.24603346
CGTATGC39300.049.7947744
GTATGCC39650.049.26571745
ATGCCGT42000.046.75739747
TCGTATG42950.045.48112543
TGCCGTC46800.042.0363648
TATCTCG48600.040.62410739
ACGTCTG50800.039.82016415
CCGATGT50500.039.78496633
GCACACG52800.038.4425111
GCCGTCT51250.038.38505649
CTCGTAT51650.038.0913342
ACACGTC54300.037.0581313
CACACGT55050.036.8080812
GATGTAT60750.033.24545335
CGATGTA61150.033.0268534
CACGTCT63350.031.98539414
AGCACAC67350.030.76109110
CGTCTGA66000.030.64990816
TCTCGTA65850.029.98255341
Produced by FastQC (version 0.11.5)