FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181712
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT274830.29932326346110616TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149520.16284544755923513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC32800.058.15971433
TATGCCG34600.054.93831648
GTCCGCA36350.052.4774534
CCGCACA37100.051.0439436
ACGTCCG37750.050.53374532
GTATGCC37900.050.24795547
CGTATGC38650.049.81716546
TCCGCAC39800.047.84292635
CGCACAT39700.047.70231637
ATGCCGT41550.046.08136749
TCGTATG42200.045.29702445
ACGTCTG43800.044.82518815
GCACACG47600.041.39104511
GTCACGT46350.041.30918529
CTCGTAT46500.041.11028344
TGCCGTC46400.041.03768550
CACACGT50450.038.9140712
GCCGTCT48700.038.883351
AGTCACG49900.038.72108528
TCACGTC51650.037.06746730
Produced by FastQC (version 0.11.5)