FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25976556
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1461440.5625995994234185TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG174600.061.13283546
CGTATGC184700.057.79211844
GTATGCC187750.056.94651445
ATGCCGT192150.055.6042547
TCGTATG193800.055.20458643
ACGTCTG211050.051.28904715
CTCGTAT210300.050.48951342
TGCCGTC215050.049.63391548
GCACACG219850.049.10618611
GCCGTCT217550.049.02899649
AGTCACG222500.048.17228
CACACGT224550.048.09431512
GTCACGA224550.047.66895729
CGATCAG226500.047.27219833
ACACGTC236550.045.6839413
CGTCTGA244600.044.1976416
CACGTCT248750.043.5421714
TCTCGTA251050.042.41793441
TCACGAT258200.041.71092630
ACGATCA260100.041.52886232
Produced by FastQC (version 0.11.5)