FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Akita_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12664867
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5508074.349094230519752TruSeq Adapter, Index 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130740.10323045634825855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG597800.067.7546846
CGTATGC604600.066.9910744
GTATGCC605800.066.874445
ATGCCGT607450.066.6657447
TCGTATG609350.066.5216343
ACGTCTG613200.066.4893115
GCACACG616500.066.340311
TGCCGTC613500.066.0085848
CACACGT620250.065.9281912
GCCGTCT616350.065.6883749
CTCGTAT611650.065.6793342
ACACGTC625800.065.3033213
CGTCTGA630450.064.6945716
CACGTCT632700.064.536614
AGCACAC637800.064.25648510
TCGGAAG639350.064.105673
TCACGAT627950.063.92634235
GAGCACA642900.063.82229
TCTCGTA629100.063.555541
CACGATC629850.063.22926736
Produced by FastQC (version 0.11.5)