FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18675266
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650469634.83054003086221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC5650.037.16894549
TCGCCGT5750.037.1323346
CGCCGTA5600.036.8761947
GCCGTAT6050.034.71121648
CACTCTT8300.032.056362
CGTATCA6750.031.11145250
CTCGGTG6800.030.87863738
TTCCCTA4100.030.7261988
TCCCTAC3950.028.3501859
TCTCGGT7750.027.54507337
GATCTCG7600.027.16771135
AGCGGTT5100.025.38862610
GGCCCGA2350.025.32044446
AGATCTC8350.023.888734
CGGTTCG3550.022.67308612
TTTCCCT6850.022.4782057
CGACGCT4400.022.26897417
CCGATCA2108.367351E-1121.66655525
GTATCAT9700.021.65186751
TGAACCG4400.020.6799429
Produced by FastQC (version 0.11.5)