FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_LB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_LB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24061586
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG150600.014.0833375
CGAATAG683800.013.74887970
TAGACCG33700.013.2934585
CTATACT84050.012.8671214
AATAGAG590750.012.85720170
TATACCG46150.012.7407655
TATACAG193250.012.5145855
TTATACT147550.012.3583124
GTATTAG86350.012.2416631
CTTAGAC65650.012.2084993
CCCTTAG42400.012.0526571
CTACACT92700.012.0440234
GAATAGA635950.012.04244770
GTATAGA118850.011.868661
GTGTAGG92350.011.7116311
ACTGTGC104950.011.6385338
TCTATAC112550.011.5991143
TAATACT133450.011.5921584
GGTGTAG162950.011.44888668
GTATAGG64000.011.3757271
Produced by FastQC (version 0.11.5)