FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_LB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_LB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22642914
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250600.1106748009553894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG751700.014.39764270
TAGACCG29300.014.3340165
AATAGAG597250.014.18845470
CTTAGAC61150.013.1641063
GAATAGA676650.012.94771770
CCCTTAG39600.012.7288071
CTATACT78800.012.5695364
TATACTG146050.012.4850525
TATACCG44800.012.1089915
TATACAG183450.012.0573985
GTATTAG81200.011.9842031
ACTGGTC76550.011.8416618
GTATAGG58500.011.6680741
ACGAATA940050.011.63226269
GTATAGA114300.011.6068241
GTATATA287150.011.568521
TAGAGAG375350.011.55397270
GGTGTAG148900.011.51872868
TCTATAC106500.011.3378183
TTATACT137850.011.2728894
Produced by FastQC (version 0.11.5)