FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_Bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_Bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24658202
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257070.10425334337029114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG47450.013.5738121
CGAATAG523550.013.37760370
TATACTG166300.012.9220425
CTTAGAC70500.012.7091233
TAGACCG33050.012.7076385
TATACAG211400.012.6155175
CTATACT87650.012.5782144
GTATAGG67900.012.4757251
CCTATAC64100.011.7939823
GTATTAG95350.011.6007631
TTATACT167550.011.4471254
GTATATA373800.011.0406511
ACTGTGC105300.011.0348078
GTATTAT213450.010.9711011
CTACACT94900.010.9165614
GTATAGA139400.010.8728981
AATAGAG529900.010.81294870
TAATACT152300.010.7778784
GGTGTAG155600.010.75271868
GTATAAG109150.010.7433881
Produced by FastQC (version 0.11.5)