FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n02_Bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n02_Bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003357
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG30200.014.834085
CCCTTAG43850.014.4485361
CTATACT76650.014.109254
CGAATAG383950.013.455440570
CTTAGAC67450.013.2837163
TATACTG142700.013.2197255
TATACAG188950.012.706765
CCTATAC55300.011.9618473
GTATTAG83850.011.9392711
CCTTAGA60500.011.9173132
GTATAGG59450.011.8347491
TAATACT133450.011.8281164
CTACACT87400.011.6530384
GTGTAGG85700.011.5181621
GTATAGA119200.011.4525781
ACAGTGC94650.011.38892658
TTATACT144700.011.3680754
GTGTTAG101800.011.31261351
TACACTG152050.011.1868415
GTCTAGG36000.011.1817621
Produced by FastQC (version 0.11.5)