FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18675266
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG617143433.046029973548976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTT15200.020.0292815
CCCTTAG3400.019.583191
ACCGATC9100.019.22992734
GGGGTTT14750.018.74192816
CGATCTC10100.017.67249136
CCGATCT9950.017.58712235
AGACCGA11150.016.63608732
GATCTCG10600.016.17870937
ACTGTGC7600.016.11568
TGGTGGG15050.015.8110587
CCCCTAT2906.1390892E-915.7092031
TAGACCG1807.153209E-515.5573325
GCTCGGA2604.8621223E-714.8103352
CTGTGCA9350.014.5968839
GCGATCG2202.4613328E-514.3161749
AAGGGGG13500.014.26237770
GTATAGG5250.014.0174421
CTAGGCT2505.268579E-614.0012994
CCTTAGA4051.8371793E-1013.8296282
ATGCCGT14300.013.70495845
Produced by FastQC (version 0.11.5)