FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_LB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_LB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24061586
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG575860.2393275322748883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG34200.015.2495625
ATCTCGT109750.015.02010770
AATCTCG110000.014.57234369
ACAGGCA506200.014.47808670
TATACCG48800.013.9148695
CTTAGAC66900.013.8647643
TATACTG151300.013.7187015
GACAGGC580650.013.15215670
TATACAG191600.013.0071375
AGACAGG751050.012.93155570
CCCTTAG44750.012.9100541
GTATATA272050.012.6772381
TACACCG43250.012.5441965
CTACACT89050.012.4993324
GTATAGG64550.012.475811
GTATAGA112100.012.3063081
GTGTTAG115250.012.2433781
CTATACT86350.012.0794614
TACACTG163800.012.0734615
TAATACT131150.011.8497264
Produced by FastQC (version 0.11.5)