FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_LB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_LB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22642914
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG583250.257586103979373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC472200.017.13613770
GATCTCG99200.016.2292469
ATCTCGT103250.015.59261170
GACCGTA590150.014.93883870
CCGTACT350550.014.87604270
CGTACTA273250.014.80637570
AGACCGT754750.014.41679470
TAGACCG27250.014.00118355
GTATAGG60600.013.6358521
TATACAG177000.013.1112795
GTATAAG92650.012.7358351
GTATATA266800.012.6381461
CCCTTAG37000.012.5861491
GTATAGA107550.012.3713221
TATACTG152650.012.1530095
CTTAGAC60250.012.1418653
GAGACCG910150.012.0398869
GTGTAGG83050.012.0156741
AGATCTC138650.011.8639768
CCTATAC55550.011.5939093
Produced by FastQC (version 0.11.5)