FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_Bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_Bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24658202
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG689470.2796108167172935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG35150.016.4306565
TATACTG172950.014.227585
CCCTTAG49750.014.146071
CATCTCG95100.013.69040969
TATACAG214700.013.1401195
AGACTCC547950.013.06187370
GTATAGG69000.012.9904351
ACTCCTG418300.012.93524770
ATCTCGT101800.012.85810570
CTATACT93800.012.8365254
GTCCTAG31550.012.7623691
GTATATA341100.012.6770461
TCCTGAG241450.012.4369370
CTTAGAC76000.012.3887873
TTATACT170400.012.3657024
TAATACT160950.012.0261344
GACTCCT444550.011.93527969
GTATTAG95250.011.873271
ACTGTGC107050.011.8682638
TACACTG172600.011.8026215
Produced by FastQC (version 0.11.5)