FastQCFastQC Report
Wed 4 Jul 2018
HVF55BGX5_n01_Bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF55BGX5_n01_Bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003357
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597510.2715540178710003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG44250.016.2214661
ACGGACT268550.014.67453370
TAGACCG29650.013.930185
TATACTG150250.013.72145755
CTTAGAC66400.013.6530653
CTATACT80050.013.6423994
GTATAGG56450.013.5840611
AGACGGA414900.012.65315670
GTATATA287250.012.5552871
TATACAG185750.012.4935015
TTATACT150200.012.374344
GTATAGA110500.012.3581031
CCTTAGA58950.012.2910192
GTATAAG91650.012.0726911
GTGTTAG100650.011.9324561
GTATTAT176700.011.86971
ACTGTGC96500.011.8597758
GACGGAC341350.011.78072969
TCTATAC107000.011.7765253
TAATACT131000.011.6763634
Produced by FastQC (version 0.11.5)