FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078225
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC71430.1406593839382855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT3050.025.0923122
AGATCTC3500.021.85496334
GAGTGTA3700.021.2822928
AGTGTAG3600.021.24850829
TCTCGGT3600.021.24767137
GTGTAGA3700.020.67422530
TGTAGAT3700.020.67361331
TTACACC550.003930089520.4619754
GTAGATC3900.020.19029232
TAGATCT3800.020.1295733
TAGGGAA3950.019.93648520
AAGAGTG3850.019.86892926
TCGTGTA4000.019.12271515
AGCGGTT3250.018.69007510
GTGTAGG4250.018.52902217
GTAGGGA4400.017.89752819
CTCGGTG4300.017.78874838
TGTAGGG4400.017.3861718
ATGTTAG800.001707216316.881961
TCTACAC800.001707778916.8811283
Produced by FastQC (version 0.11.5)