FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27391041
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT146300.023.92959445
CTGACGC264700.019.63487445
TCTGACG357000.014.9428545
ATCTGAC456850.011.7557144
TATACAC1668300.011.13167245
ATACACA1270750.010.26037945
CATCTGA533600.010.04771243
GTCTTAG25600.09.9359631
TTATACA2131700.09.75988945
CTATACT52400.09.7081574
GTCTAGG31200.09.5955211
GTATAGG49000.09.46331
GTGTAGA49700.09.3753051
GTATTAG42950.09.2240361
ACTGTGT43200.09.218258
TAGACTG32450.09.1562595
TATACCG42650.08.9719885
GGACCGT47900.08.971336
GTCCTAG33150.08.9631761
TATACTG58850.08.9501065
Produced by FastQC (version 0.11.5)