FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23890758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT118450.022.60368745
CTGACGC210400.018.02931645
TCTGACG273950.014.54503145
TATACAC1280450.011.64452445
ATCTGAC349400.011.46854844
ATACACA961450.010.64292945
GTGTTAG33400.010.3115551
GTCCTAG29900.010.16346551
GTATAGG38800.010.1527941
TTATACA1652450.010.08376645
GTATTAG35100.09.9403981
CATCTGA404950.09.872931543
GTGTAGG42300.09.525591
GTCTAGG27900.09.3590771
TAGACAG28800.09.3008145
GTATAAG37600.09.1597341
ACTGTGC45200.09.1587818
CTATACT47200.09.156444
TATACTG47900.09.0226115
TAGACTG28250.08.9241335
Produced by FastQC (version 0.11.5)