FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595842
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT102700.022.06120945
CTGACGC172750.016.2412245
TCTGACG216700.013.66366745
TATACAC1127850.012.94683545
ATACACA813900.011.64084445
TTATACA1491050.010.97014845
ATCTGAC278200.010.72399644
TAGACAG27550.010.7032365
TACACCG43300.09.8771385
GTATAGG43050.09.7256381
GTATTGG55950.09.6558271
GTGTTAG37200.09.6212721
GTGTAGG45200.09.5120311
GGACCGT44500.09.403976
CATCTGA319100.09.35630243
ACTGTGC43700.09.3187038
TATACTG48850.09.2618325
ACCGTCC61850.09.0940778
CTTATAC1848300.09.07375845
CTATACT48350.09.0317244
Produced by FastQC (version 0.11.5)