FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25846712
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT117400.023.8025445
CTGACGC227350.019.17911545
TCTGACG299900.015.07208245
ATCTGAC390150.011.62019144
TATACAC1342950.011.20317945
GTCTTAG24150.010.6257821
GTGTAGG49050.010.4174171
ATACACA1022750.09.99188545
CATCTGA452650.09.98568843
GTCCTAG35600.09.863881
TTATACA1721350.09.76254645
GTATTAC38800.09.45647051
GTATAGG44750.09.40636351
ACCGTGC71050.09.3415048
GGACCGT39400.09.3078696
GTGTTAG41650.09.2417521
GTATTGG61450.09.2310661
CTACACT45850.09.2295554
GTATTAG43000.09.2133461
TAGACAG32100.09.1860275
Produced by FastQC (version 0.11.5)