FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n02_ATAC_Root_Aug_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43450501
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT316150.021.79843945
CTGACGC534300.017.40411245
TCTGACG636950.015.54596245
ATCTGAC802850.012.3643944
TATACAC2861050.011.75203345
ATACACA2127250.011.05695645
CATCTGA902800.010.97031643
GTATAGG71850.010.2444951
TACACCG77100.010.2181495
GTGTAGG68350.010.04455951
TTATACA3678350.09.97453445
TATACCG62550.09.8960975
TAGACTG38000.09.8329325
GTATAGA66800.09.7384761
ACATCTG1033750.09.73518142
TATACTG58750.09.7316045
GTGTAGA70900.09.6832961
GTATTGG80200.09.570831
GTCTAGG39950.09.5222481
ACTGTGC56000.09.4816328
Produced by FastQC (version 0.11.5)