FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078225
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105640.20802544196052755No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC84100.165609046468008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC2850.027.63771845
CTCGTAT3700.021.28138540
CCGAGAC3600.021.2549329
GAGACCG3650.020.34959431
TCTCGTA3800.020.1293139
ACCGATC3750.019.79893934
CTCCGAG4750.019.42536745
AGACCGA3950.018.80312732
ATATCGT600.00651624718.7486276
GATCTCG4100.018.65496337
GTAATAC851.205238E-418.5298793
TATACTT851.205238E-418.5298795
ACACCGT750.001111480617.998686
AGGAATG4800.017.81540722
GGAATGC4850.017.6317423
CGAGACC4250.017.4767130
ATGCCAA800.001714127716.87176911
GCATTAT952.8116725E-416.5867161
TAGAGCT952.8211952E-416.5793634
CGGTTCA5350.016.39808313
Produced by FastQC (version 0.11.5)