FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27391041
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC145550.023.88220845
CTCCGAG252400.020.52876345
TCTCCGA382300.014.11247245
ATCTCCG477750.011.3118344
TATACAC1676350.011.13830945
GTCTTAG22700.010.3145521
ATACACA1277050.010.28523945
GTATAGG44450.09.82591151
TTATACA2142750.09.75234145
GTGTAGG49500.09.6876241
GTATTAG44150.09.63771251
TAGACCG29000.09.4680635
GTATAGA49700.09.3768471
TACACCG53250.09.2982755
GTCCTAG32350.09.2559351
GGACCGT49050.09.2211826
GTATAAG43250.09.161561
TATACTG57250.09.0810445
CTAGGAC33250.09.0701083
CTATACT53800.08.9522224
Produced by FastQC (version 0.11.5)