FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23890758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC112550.023.88798345
CTCCGAG190500.019.91221845
TCTCCGA291200.013.75262945
TATACAC1283900.011.63924345
ATCTCCG367350.010.90186144
GTGTAGG40850.010.7471721
ATACACA963250.010.6484845
TTATACA1660700.010.09981745
GTCCTAG29600.09.7357611
GTGTAAG29050.09.6875851
TATACTG45700.09.5047545
GTATAGG41150.09.3557351
GTATAGA38200.09.2531151
TAGACTG29850.09.1984635
CTATACT47450.09.0119164
TAGACCG24650.08.9476495
TACACTG41300.08.93703655
GTATTGG53500.08.9214141
ACCGTCC63050.08.8496198
ACATCTC457500.08.82237842
Produced by FastQC (version 0.11.5)