FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595842
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC104450.021.58323745
CTCCGAG159850.017.55137345
TATACAC1128200.012.94442745
TCTCCGA236950.012.67598845
ATACACA820000.011.60603245
TTATACA1498300.011.00833745
ATCTCCG289050.010.39906944
GTATAGA45650.010.2582111
TATACTG53050.09.9272625
GTGTAGG42750.09.6901571
GGACCGT39800.09.6114826
GTATAAG39450.09.5876331
TAGACTG27100.09.5505325
GTGTTAG35700.09.4595861
GTCCTAG29350.09.4350911
TATACCG33350.09.3128425
TACACCG45200.09.3111255
GTATTGG51600.09.2934941
GTATAGG39650.09.2553641
GTCTAGA32800.09.1977181
Produced by FastQC (version 0.11.5)