FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25846712
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC115250.024.2067345
CTCCGAG209200.020.87465345
TCTCCGA317850.014.28414945
ATCTCCG395550.011.501144
TATACAC1345300.011.2267245
TAGACAG34000.010.3924545
GTATAGG46850.010.284341
GTCTTAG25050.010.1564741
ATACACA1027400.010.02297845
TTATACA1723350.09.77439145
TATACTG55650.09.6251875
GTATAGA48250.09.5193011
TACACCG47800.09.3696345
GTATTGG58000.09.2389191
GTCTAGA36550.09.1168861
ACATCTC502800.09.07908742
AGACCGT34050.08.9876146
GTATTAG44350.08.9349331
TAGACTG32050.08.9181165
GTGTAAG32700.08.882071
Produced by FastQC (version 0.11.5)