FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02_n01_ATAC_Root_Aug_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43450501
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC245950.027.88197345
CTCCGAG431150.021.47320245
TCTCCGA666750.014.96194445
ATCTCCG827450.012.05899944
TATACAC2866500.011.77320545
ATACACA2133600.011.07741545
TATACCG59100.010.9672525
TATACTG56850.010.0553245
CATCTCC1001600.010.04532243
TTATACA3687150.09.99245945
GTATAGG72750.09.9339711
ACATCTC1004800.09.91704842
GTGTAGG67550.09.7987991
GTATAGA66100.09.6390851
TACACTG52800.09.6331285
GTATAAG49300.09.4987681
GTCTTAG27850.09.4582651
CTATACT58400.09.441614
TACACCG78050.09.4002085
GTGTTAG37550.09.3533131
Produced by FastQC (version 0.11.5)