FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5025923
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA4200.029.99767328
AGTGTAG4300.028.77683429
GTAGATC4600.027.38727232
AGATCTC4550.027.19379634
TAGATCT4550.027.19379633
TCTCGGT4600.026.89821437
CACTCTT2400.026.2714842
GTGTAGA4950.024.99756430
TAGGGAA5100.024.26137520
TGTAGAT5200.024.22720131
AAGAGTG5250.023.569626
CTCGGTG5250.023.56819338
TCGTGTA5200.023.36194415
GATCTCG5350.023.12743435
GTAGGGA5600.022.4969119
GTGTAGG5500.021.67862517
TGTAGGG5700.021.31286218
TCGGTGG6050.020.4519439
GTATAGA908.199324E-620.0171681
AGCGTCG6600.019.09037611
Produced by FastQC (version 0.11.5)