FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26724605
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT142450.024.05333145
CTGACGC263950.019.40792545
TCTGACG352100.015.16884445
ATCTGAC447150.011.98467644
TATACAC1654450.011.4878345
ATACACA1247500.010.40213445
CATCTGA520750.010.29946743
GTCTTAG21850.010.2032891
GTGTAGG48100.010.0658361
TACACCG49250.09.6470475
TTATACA2105300.09.55561145
ACCGTGC72750.09.4635818
GTATAGG44600.09.4419811
TAGACAG30700.09.3150155
GGACCGT47500.09.2851136
ACATCTG606400.09.111670542
GTATTGG63350.09.029081
GTCTAGG29450.09.0230461
GTATAAG43600.08.9870921
CTATACT54450.08.9738924
Produced by FastQC (version 0.11.5)