FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310831
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT114500.022.890545
CTGACGC209650.018.62900745
TCTGACG277300.014.83879745
ATCTGAC356100.011.61835444
TATACAC1283050.011.51134245
ATACACA963400.010.59723845
TTATACA1651150.010.03372245
CATCTGA413100.09.99345643
GTGTAAG27750.09.8191271
GTCCTAG30450.09.6880081
GTATAGG40500.09.5636571
TAGGACT30650.09.2567374
AGACCGT34000.09.1333296
GTATTAG36350.09.10675051
GTCTAGA27700.09.1051851
GTATAGA38700.09.07745551
GTATAAG36350.08.9828491
ACATCTG475350.08.92597342
GTGTAGG42450.08.9121421
GTGTTAG33150.08.898941
Produced by FastQC (version 0.11.5)