FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23037944
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT102500.021.22445545
CTGACGC168700.016.44303345
TCTGACG210250.013.45033845
TATACAC1109400.012.5526845
ATACACA817150.011.75601345
TTATACA1468550.010.99482245
ATCTGAC273150.010.42717144
TATACTG51100.010.1793315
GTATTGG49700.010.013781
TACACCG43950.09.6834725
GTATAGG41500.09.659041
GTCCTAG28950.09.4123681
TAGACCG26000.09.3535955
CTTATAC1830400.09.20105845
TAGACAG27950.09.1844085
TACACTG42850.09.1437975
GTATTAG37450.09.14016151
TACACAG33600.09.1143935
CTATACT50350.08.9445724
TACACAT598700.08.93962145
Produced by FastQC (version 0.11.5)