FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25239059
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT117100.023.41983845
CTGACGC219200.018.90541345
TCTGACG293000.015.38746945
ATCTGAC383050.011.82881144
GTGTAGG47850.011.2946031
TATACAC1342400.011.20860145
CATCTGA446550.010.16689243
ATACACA1024050.010.0641345
GGACCGT40700.09.8965626
TTATACA1722050.09.8545245
GTATAGG45300.09.7928641
TACCCCG27750.09.4936195
GTCCTAG34450.09.2819831
ACTGTGC51450.09.1396568
CTAGGAC33450.09.087783
ACATCTG515850.09.04074942
GTGTAGA48850.09.0351041
GTGTAAG33150.09.0346171
GTATTGG54500.08.8834911
GTATAAG43350.08.8827751
Produced by FastQC (version 0.11.5)