FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n02_ATAC_Root_Aug_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42606396
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT318800.021.99664345
CTGACGC540500.016.79522145
TCTGACG634100.015.26692145
ATCTGAC793650.012.23461444
TATACAC2833050.011.83787845
ATACACA2102350.011.0682645
CATCTGA888850.010.92929343
TACACCG74850.010.829585
TATACCG60450.010.3177365
GTATAGG71800.010.2242831
GTGTAGG64950.010.1931361
TTATACA3659850.010.12560445
TATACTG55950.09.9805055
ACTGTGC55400.09.8688148
GTGTAGA70900.09.7506111
GTATAGA64700.09.7104561
ACATCTG1024900.09.62320642
ACCGTGC161500.09.4733788
CTAAGAC27050.09.240013
GTATTAG44000.09.1097611
Produced by FastQC (version 0.11.5)