FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5025923
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG499120.9930912192646006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC5000.026.10254529
GAGACCG4950.025.91161731
GATCTCG4950.025.90852437
AGACCGA5000.025.65224632
TCTCGTA5100.025.5874239
CTCGTAT5050.025.39523140
CGAGACC5100.025.14951130
TCCGAGC2600.025.09410545
ACCGATC5150.024.90385434
CGATCTC5500.023.72698836
CCGATCT5750.023.08668135
GGAATGC5700.022.89309723
ATCTCGT5750.022.30385838
ATGCCGT5900.022.11683845
CTCCGAG4500.021.99820345
AGGAATG6000.021.74844222
GTGTTAC550.00392457920.4669271
GACTAGC550.003938629820.45430235
GGCCTAG807.5343574E-519.6994171
AGATCGG6650.019.2973881
Produced by FastQC (version 0.11.5)