FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26724605
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC137600.024.64049545
CTCCGAG245300.020.74673345
TCTCCGA375500.014.296020545
ATCTCCG470650.011.46319844
TATACAC1659450.011.46044145
ATACACA1253200.010.36599545
GTATAGG45450.010.1540761
GTGTAGA47650.09.9214881
GTGTAGG45550.09.6375491
ACCGTCC69150.09.6306358
AGACCGT40300.09.6043926
TTATACA2109650.09.54907545
GTCCTGG56800.09.3933491
ACATCTC586000.09.20290842
GTATAGA46650.09.120751
CATCTCC599100.09.09931643
GTATAAG42900.09.0783991
GTATTGG61150.09.0196581
TACCCCG31600.08.9019945
TCTATAC46100.08.8358363
Produced by FastQC (version 0.11.5)