FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310831
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC106650.024.40764445
CTCCGAG187250.020.76229145
TCTCCGA291950.014.22579645
TATACAC1286950.011.49443145
ATCTCCG367500.011.36862544
ATACACA974600.010.5266945
GTATAGG40700.010.4542941
GTGTAGG43500.010.4541661
TTATACA1654500.010.03151145
TACACTG40850.09.8059235
TACACCG45350.09.5772445
GTATAGA39350.09.5543031
TATACTG47000.09.5283075
ACCGTCC64000.09.4915918
GTATTGG52200.09.4449781
GTGTAAG28150.09.2769861
GTATTAG34100.09.1767251
GGACCGT40000.09.1700426
ACATCTC458900.09.09450842
GTATAAG39400.09.0850691
Produced by FastQC (version 0.11.5)