FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23037944
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC101900.021.32836745
CTCCGAG155950.017.4996845
TCTCCGA224700.012.71614245
TATACAC1108750.012.55657945
ATACACA814650.011.8202745
TTATACA1471900.010.96879345
ACTGTGC41800.010.4419598
GTGTAGG45600.010.1206111
ATCTCCG287550.09.99935944
GGACCGT37350.09.88096
GTCCTAG30350.09.7169581
GTATAAG37900.09.6820241
ACTGTCC34650.09.6098168
CTACACT36900.09.4530554
TACACTG42250.09.3211755
TATACTG50800.09.3028265
GTATAGA41450.09.2329851
CTTATAC1830950.09.21470745
ACCGTCC61950.09.0430438
TACACAT597600.08.96779845
Produced by FastQC (version 0.11.5)