FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25239059
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC114300.024.07322145
CTCCGAG201450.020.393345
TCTCCGA311100.014.51447545
ATCTCCG390650.011.59913644
TATACAC1340300.011.21988545
TAGACTG33650.010.2322145
ATACACA1023800.010.08455945
TTATACA1719550.09.88100645
GTGTAAG32100.09.7485891
TATACTG56450.09.6873645
GTGTAGG46450.09.5480021
TACACCG45000.09.3017295
TATCCCG34750.09.26071555
GTATAGG45100.09.18487551
GTACAGA50550.09.1298711
TACACTG45900.09.1193425
ACATCTC503100.09.03786842
GTATTAG42400.09.0264091
GTCCTAG30700.09.0198371
GTGTTAG41300.08.9942691
Produced by FastQC (version 0.11.5)