FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01_n01_ATAC_Root_Aug_Rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42606396
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC246200.028.3013745
CTCCGAG426850.021.05696745
TCTCCGA654700.014.79741945
ATCTCCG812800.011.93020444
TATACAC2832300.011.83748945
ATACACA2104250.011.07044745
TATACTG54050.010.9917685
TACACCG72850.010.5646685
GTGTAGG63550.010.34394551
TATACCG60550.010.3321315
TTATACA3657250.010.14364345
CATCTCC983600.09.92488443
ACATCTC990850.09.81139342
GTATAGA61550.09.8022471
CTATACT58700.09.6994674
GTCCTAG36750.09.6174781
GTCCTAC48250.09.6114471
GTGTAAG30450.09.6111411
GTATAAG46050.09.5817611
GTATAGG67800.09.5295221
Produced by FastQC (version 0.11.5)