FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922218
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3947015.7019440878631675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG498490.7201304552962648No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70240.10147036686796052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT3150.032.581682
TATCACA352.8944627E-432.0038766
TACAGTC300.00535837929.7563953
TAGATCT3750.024.6455633
TCTATAC551.4470443E-424.3461423
ATACTGC751.8246083E-623.8051173
AGATCTC3900.023.69952434
TGCCTTA702.790627E-522.4099187
TCACTAG602.5426951E-422.4027126
TACCCTA500.002335591722.38275
CTAAGCG500.002358328622.3454064
TTATCCG602.587147E-422.3454044
TCTAGGC500.00237563522.3172973
GTACAAT500.0024083822.2647631
TGTAGAT4300.021.49780331
AGTGTAG4200.021.47232629
TCCCTAC2200.021.4572759
ATAGTCC754.9054906E-520.8294773
GTTCTAT754.99535E-520.7804451
TCCAATG550.004025547820.3774498
Produced by FastQC (version 0.11.5)