FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922218
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4294376.203748567294471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCC208.4474904E-443.3596653
ATATGTC250.002529019534.6877333
GACTATC353.2397016E-431.3949097
GTCCTAC353.7948354E-430.5592021
CTTATGT300.00555178729.5413289
AACTTAG454.102143E-529.3019147
AGAACTA300.00590545129.170365
GTTATGA300.006590890728.5219231
CTCCGAG7350.026.16095545
GGATAAG501.0100892E-425.6697311
TTGAAAA1950.025.42043318
CTCGTAT4300.023.93779240
CTTGAAA2150.023.01182217
TACTGGG752.4962592E-622.9621452
GACAACT1053.4942786E-922.7952444
ACCGATC4300.022.29172534
ACTGTGC500.00251898422.0932948
TAGGTAG500.002666515521.877775
TACTCTG500.002666515521.877775
TACGATC500.002752043521.7590984
Produced by FastQC (version 0.11.5)