FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02_n01_ATAC_Root_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02_n01_ATAC_Root_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28989798
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG520200.027.50493645
TCCGAGC218500.025.17790245
TCTCCGA870700.017.93424445
ATCTCCG1094800.014.28705944
GTGTAAG16200.013.6187431
GTCTAGT6500.013.5075691
CATCTCC1245200.012.59213543
GTCTAGA8650.012.492551
CTAGACT8750.012.3443024
GTGTAGA39200.012.0028991
AGACTAG9050.011.93258825
ACATCTC1315400.011.87220142
GTATAAG14900.011.7851281
TACACCG96200.011.5787985
TAGACAG8950.011.5655995
GTGTAAC18550.011.1652871
GTCCTAC23000.011.0605451
GTTCTAG10400.011.0398411
GTGTAGG44050.011.03910351
GTCTAAG8050.010.9067331
Produced by FastQC (version 0.11.5)