FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02_n01_ATAC_Root_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02_n01_ATAC_Root_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31735216
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC327750.030.06958445
CTCCGAG816350.027.79133445
TCTCCGA1354100.017.7483245
ATCTCCG1688450.014.24419144
ACTCCTA16700.013.47262431
GTGTAAG14800.012.7772321
CATCTCC1914850.012.581243
ACATCTC2034200.011.809834542
GTCTTAG7550.011.3306771
GTACTAG5600.011.2561331
CACATCT2208000.010.87116941
GTATAGA18100.010.8208121
TACACCG87450.010.6529895
CTACACT11550.010.5206544
GTATAAG16750.010.4833231
GTCTAGA7800.010.3902761
GTCCTAC25800.010.2963071
GTGTAGA44800.010.1003691
TCGTATG23450.09.97983740
GTACAAG54200.09.9685321
Produced by FastQC (version 0.11.5)