FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6660584
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3529455.299009816556626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG665120.9985911145328998No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112140.1683636149622916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT3750.030.929652
TGTAGAT4300.028.81036831
AGATCTC4350.028.48938834
TAGATCT4450.028.35598633
AGTCTAA406.2907283E-428.0313556
ACAGTAC507.473747E-526.8322563
GTAGATC4950.025.94400432
ATTCAAC957.9853635E-1025.8907723
GAGTGTA4700.025.88001828
CTTATCT450.00126195824.8803674
TTCCCTA2050.024.11318
ACTCTCT751.7201492E-623.9669598
AGTGTAG5300.023.37634729
ACTATTA500.00228393122.4690278
TAGAACT500.002329754922.392334
AGTATAC500.002349262422.3602143
TTACAAT500.00238767722.2978671
TAGGGAA5550.021.90396520
AAGAGTG5700.021.73484226
TCTCGGT5700.021.35023337
Produced by FastQC (version 0.11.5)