FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01_n02_ATAC_Root_Rep3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01_n02_ATAC_Root_Rep3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28261848
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC608500.023.16648745
TGACGCT264300.020.52054445
TCTGACG824100.018.77148445
ATCTGAC1023200.015.12936644
GTGTAAG14450.014.476961
CATCTGA1157200.013.38715743
GTCCTAG8050.012.2947321
ACATCTG1301150.011.94393642
GTCTAGA7600.011.8388271
CTACACT12200.011.8000314
TACACTG13650.011.700165
GTATAGG17450.011.3435631
GTGTTAC17100.011.3126561
GTATAGA17300.011.1818741
GTGTAGG40050.011.0643531
GTTCTAG11800.011.056261
CACATCT1420400.010.99962341
GTGCTAG13300.010.9931961
TTACACT10650.010.9828934
GTGCTAC29000.010.8590621
Produced by FastQC (version 0.11.5)